Coding

Part:BBa_K2032008:Design

Designed by: Chun Mun Loke   Group: iGEM16_UMaryland   (2016-10-12)


sMMO and GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5641
    Illegal NotI site found at 3792
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3102
    Illegal BamHI site found at 3136
    Illegal BamHI site found at 6160
    Illegal XhoI site found at 6061
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 110
    Illegal NgoMIV site found at 1021
    Illegal NgoMIV site found at 1199
    Illegal NgoMIV site found at 3749
    Illegal NgoMIV site found at 4585
    Illegal AgeI site found at 3996
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1160
    Illegal BsaI site found at 1225
    Illegal BsaI.rc site found at 1268
    Illegal BsaI.rc site found at 1724
    Illegal BsaI.rc site found at 2111
    Illegal BsaI.rc site found at 2249
    Illegal BsaI.rc site found at 2666
    Illegal BsaI.rc site found at 3815


Design Notes

Gibson and Ligation


Source

M. capsulatus et al.

References