![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K2032008:Design
Designed by: Chun Mun Loke Group: iGEM16_UMaryland (2016-10-12)
sMMO and GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 5641
Illegal NotI site found at 3792 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3102
Illegal BamHI site found at 3136
Illegal BamHI site found at 6160
Illegal XhoI site found at 6061 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 110
Illegal NgoMIV site found at 1021
Illegal NgoMIV site found at 1199
Illegal NgoMIV site found at 3749
Illegal NgoMIV site found at 4585
Illegal AgeI site found at 3996 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1160
Illegal BsaI site found at 1225
Illegal BsaI.rc site found at 1268
Illegal BsaI.rc site found at 1724
Illegal BsaI.rc site found at 2111
Illegal BsaI.rc site found at 2249
Illegal BsaI.rc site found at 2666
Illegal BsaI.rc site found at 3815
Design Notes
Gibson and Ligation
Source
M. capsulatus et al.